Abubuwan gwaji don acetone a cikin fitsari: umarnin don amfani, farashi
Kayan Alurar Acetone Gwaji - Tsarin tsarin bincike wanda ke amsa gawar ketone kuma yana nuna sakamakon binciken ta canza alamomin launi. Idan ya cancanta, mai haƙuri na iya siyan su a kantin magani.
An tsara matakan don auna matakan jikkunan ketone a cikin fitsari. Addamar da acetone da abubuwan da ke tattare da ita yana ƙaruwa tare da cututtukan kumburi, cututtukan cututtukan ƙwayar jijiyoyi, matsananciyar yunwa da sauran yanayi. Koyaya, a cikin aikin asibiti, yawanci ana amfani da gwajin don bin diddigin ciwon sukari. Rashin kula da cutar na kara yawan ketones a cikin fitsari.
Ka'idojin aiki
Abubuwan gwaji alamun nunawa ne na yawan ketones a cikin fitsari. A ƙarshen ƙarshen su akwai shafin cike da sodium nitroprusside. Idan aka haɗu da acetone, abu yana canza launi.
Kafin amfani, abubuwan farin suna da fari. Bayan hulɗa tare da ketones, wani launi mai launin violet ya bayyana. Colorarfin launi shine daidaita kai tsaye zuwa adadin acetone a cikin fitsari.
Don yanke hukunci, ya kamata ku kwatanta inuwa na tsiri tare da ma'aunin launi da aka haɗe. Smallestarancin ƙaramin bincike shine 0,5 mm / L. Kadan jikin ketone a cikin fitsari baza'a iya tantancewa ta amfani da gwaji ba.
Ma'anar Scale
Yin amfani da gwajin, wanda zai iya yin hukunci ba kawai kasancewar jikin ketone ba, har ma da matsayin girman karuwarsu. Sabili da haka, ana amfani dasu don madaidaiciyar hanyar.
A bisa tsari, ana iya raba sakamakon binciken zuwa rukunoni biyar. A yadda aka saba, abubuwan bai da launinsu, wannan yana nuna rashin acetone a cikin fitsari. Ana lura da sakamako mara kyau yayin da adadin jikin ketone ya ƙasa da 0.5 mmol / L.
Ana lura da launin ruwan hoda mai haske tare da ƙara ƙaru a cikin ketone a cikin fitsari. A aikace, an ayyana shi azaman da ƙari. Wannan yanayin ana kiranta mai laushi ketonuria. Ba barazanar rayuwa bane ga mai haƙuri, amma yana buƙatar ganewar asali da magani.
Launi mai ruwan hoda da rasberi shine sakamakon ƙaruwa mai ƙarfi a matakin ketone jikin. An nuna shi ta biyu ko uku, bi da bi. Wannan gwajin launi yana nuna tsananin zafin ketonuria. Halin yana buƙatar magani na gaggawa, yana da haɗari ga lafiyar mai haƙuri.
Ana lura da launi na violet tare da ƙaruwa mai ƙarfi a matakin acetone a cikin fitsari. A aikace, wannan launi gwajin yayi daidai da ƙari huɗu. Hoton launin shuɗi yana faruwa ne sakamakon tsananin ƙarfin ketonuria - ketoacidosis. Halin yana da haɗari ga rayuwar mai haƙuri, yana buƙatar magani cikin gaggawa a asibiti.
Kayan Ketogluk-1 sune filastik tare da abubuwan abubuwan firikwensin guda biyu. Na farkon su ke tantance matakin glucose, na biyu - adadin acetone a cikin fitsari. An tsara hanyoyin gwaji don bin diddigin ciwon sukari. Bayan buɗe marufi, ana iya amfani dasu tsawon watanni biyu.
Ketogluk-1 suna da matsakaicin tsada, a cikin kunshin ɗaya ya ƙunshi rabe 50. Rayuwar shelf shine shekaru 2. Hankalin gwajin ya dogara da ingancin ma'aunin. Sakamakon ƙarya yana iya alaƙa da amfani da wasu magunguna, gurɓataccen abinci a kan jita-jita.
Don saurin kamuwa da cutar sankara, mara lafiyar yana buƙatar tattara matsakaitan fitsari. Ana samun ingantaccen sakamako a cikin nazarin fitsari na safe. Ya kamata a tattara a cikin tsabtataccen abinci waɗanda ba su da magunguna a farfajiya. Za'a iya amfani da fitsari sabo ne kawai don aunawa.
Dangane da umarnin don amfani, saukar da tsiri a cikin fitsari don 5 seconds. Sannan zaku cire ragowar ruwa daga ciki tare da kaɗawar hannunka, sanya shi a kan tebur tare da firikwensin sama. Bayan dakika 120, mai haƙuri na iya kimanta sakamakon binciken.
A yadda aka saba, alamar tsiri ta gwajin ba ta canza launi. Tare da karuwa da sukari na jini, launinta ya zama launin kore, sannan shuɗi, sannan kuma kusan baki. Babban matakan glucose yana nuna mellitus na ciwon sukari da rushewarsa, matsanancin ƙwayar cuta ko ƙwanƙwasa ƙwayar cuta, da ciwan ciki. Tare da haɓakar acetone, alaƙar kwalin ya zama ruwan hoda sannan ya zama mai shunayya.
Ketofan sune madaukai tare da mai nuna alama don sanin matakin acetone a cikin fitsari. Rayuwar shelf shekara biyu kenan. Kunshin ya ƙunshi fannoni 50. Ketofan gwajin yana da matsakaicin tsada. Bayan buɗe ginin an ba da izinin amfani dashi a cikin kwanaki 30.
Abubuwan gwaji suna amsawa da sauri ga matakan urinary acetone. Wannan shine dalilin da ya sa Ketofan galibi ake amfani dashi don lura da yanayin ciwon sukari a cikin yaro. Don bincike, zaku iya amfani da fitsari kawai da aka cakuda shi sosai.
Don sanin matakin ketone jikin, yi amfani da waɗannan umarnin:
- Cire tsiri daga batun fensir ku rufe sosai.
- Rage tsiri a cikin fitsari na tsawon dakika biyu.
- Cire tsiri daga kwano tare da fitsari.
- Zana tsiri tare da gefen kwanon ruwan don cire ruwa mai wuce haddi.
- Kimanta sakamakon bayan 2 seconds.
Manazarta suna fararen fari. Ya danganta da adadin acetone, launinta yana canzawa daga ruwan hoda mai haske zuwa shuɗi mai duhu. Gwajin yana da cikakken ƙayyadaddun bayanai, launi na tsiri zai iya ƙayyade kimanin adadin ketone jikin.
Gwajin Acetone
Acetontest alama ce ta tantance jikin ketone a cikin fitsari. An sayar da su a cikin fakitin filastik na 25 ko 50. Rayuwar shiryayye na abubuwan gwajin shine shekara guda. Bayan buɗe marufi, ana iya amfani dasu a cikin kwanaki 30. Kudin gwajin acetone shine mafi ƙanƙanta a tsakanin analogues.
Umarnin don amfani da shi don gwajin acetone ya haɗa da matakai da yawa na ganewar asali:
- Aara sabon matsakaiciyar fitsari a cikin kwano mai ruwa.
- Cire mai bincike daga bututu, sannan rufe shi da kyau.
- Nutsar da fitsari har tsawon dakika 8.
- Cire gwajin daga jirgin tare da fitsari, girgiza shi don kawar da yawan ruwa mai yalwa.
- Saka da mai nuna alama a busasshiyar kwance a sama.
- Kimanta sakamakon bayan mintuna 3.
Wani fasalin tsararran gwajin shine mafi karancin hankali zuwa karamin karuwa a jikin ketone idan aka kwatanta da analogues. Suna nuna karkacewa kawai lokacin da acetone maida hankali ne akan 1 mmol / L.
Idan babu acetone a cikin fitsari, tsirin ya kasance fari fari. Slightan ƙarami yana nuna ta da ruwan hoda mai ruwan shuɗi. Increasearin ƙaruwa a matakin ketone jikin yana haɗuwa da launin shuɗi mai tsiri.
Ka'idar aiki na gwajin gwaji "Acetontest":
Uriket-1 sune gwanon da suke da alamomi guda. Ana amfani dasu don sanin matakin ketone jikin a cikin fitsari. Mai ƙididdigar yana da cikakken ƙayyadaddun abu da azanci, yana ƙayyade mafi ƙarancin acetone a cikin fitsari.
Ana sayar da Uriket-1 a cikin kantin magunguna na 25, 50, 75 da 100. Rayuwar rayuwar shiryayye shine shekara biyu. Gwajin ƙwayar cuta yana da farashi mai araha. Bayan buɗe marufi, ana iya adana su ba fiye da kwanaki 60 ba.
Mafi yawan alamomin adadin ketones ana samunsu ne da safe fitsari. Don samun sakamako mai kyau, yi amfani da jita-jita mai tsabta ba tare da samfuran tsabtatawa ba.
Ana tsoma tsiri a cikin kwano tare da fitsari na awanni 5. Sa’annan an girgiza shi don cire yawan fitsari. Yin nazarin sakamakon zai iya yuwuwa bayan 7 seconds. A yadda aka saba, tsiri ya zama fari. Launi mai ruwan hoda yana nuna ƙara ƙarin acetone. Launin violet na gwajin yana nuna ƙaruwa mai ƙarfi a cikin adadin ketone jikin a cikin fitsari.
CITOLAB 10
Ana amfani da tsaran gwaji don tantance matakin jikin ketone a cikin fitsari. Suna da farashi mai girma idan aka kwatanta da takwarorina. Wani fasalin sananne na Citolab 10 shine yiwuwar amfanin su na shekara biyu bayan buɗe kunshin.
A kan sayarwa akwai fakiti 50 da 100. Ba a cika wakiltarsu a cikin magunguna na Rasha ba. Citolab 10 ya dace don lura da cututtukan cututtukan fata tare da haɓaka matakan ketone.
Jagororin don amfani da rari sun haɗa da matakai da yawa:
- Urineara fitar fitsari safe safe a cikin abinci mai tsafta.
- Sannan ya kamata a saukar da mai nazarin cikin fitsari har na tsawon dakika shida.
- Cire ragowar fitsari daga mai nuna alama ta girgiza kai da hannu.
- Kimanta sakamakon bayan dakika 10.
A yadda aka saba, tsiri ba ya canza launi. Increasearin ƙarami a jikin ketone a cikin fitsari yana haɗuwa da launin ruwan hoda mai haske. Tare da ƙaruwa mai ƙarfi na acetone, ana lura da launi na violet na tsiri gwajin.
Menene saurin gwajin?
Glucose shine mai samar da makamashi na jiki ga jiki, saboda rarrabuwa, mahimmancinmu yana da goyan baya, kuma an tabbatar da aikin gabobin. Tare da karancin carbohydrates a abinci, karuwar bukatar makamashi, rashi ko karancin insulin, alamar insulin juriya, isasshen glucose ya shiga cikin sassan jikin mutum, don haka jiki ya fara ciyar da sinadarai da kitse.
Rushewar kitse koyaushe yana tare da sakin jikin ketone, wanda ya hada da acetone. Mutun baya lura da karamin ketones, ana iya samun nasarar fitsari a fitsari, numfashi, da gumi.
Yawan wucewar sassan ketone yana yiwuwa tare da aiki mai aiki, mummunan aikin koda, rashin ruwa. A lokaci guda, mutum yana jin alamun guba: rauni, amai, zafin ciki. Acetone yana da sakamako mai guba a kan dukkanin kyallen takarda, amma ya fi haɗari ga tsarin juyayi. A cikin lokuta masu tsauri, saurin haɓakar jikin ketone na iya haifar da cutar ketoacidotic.
Idan acetone ya haɗu a cikin jini, ba tare da faɗakarwa ba ya shiga fitsari. Gwajin gwajin yana ba ku damar gano ainihin kasancewar ketones, ta hanyar rufe shi zaku iya yanke hukunci game da kusancinsu.
Rashin damuwa wanda zai haifar da kasancewar acetone a cikin fitsari:
- kasawa na wucin gadi na yara. Mafi yawan lokuta ana lura da su a cikin jarirai masu ƙwazo. Matsayin jikin ketone a cikinsu na iya girma cikin sauri, yana haifar da maye mai guba, don haka yana da mahimmanci a gano kasancewar su a farkon matakin,
- guba a farkon lokacin daukar ciki,
- ciwon suga
- cututtuka masu yaduwa saboda rashin abinci mai gina jiki ko cutar sankara
- zazzabi hade da rashin ruwa,
- tsaftataccen abincin carb, gajiya,
- dysfunction na pituitary gland shine yake,
- raunin da ya faru, lokacin aiki,
- wuce haddi insulin, wanda za'a iya haifar dashi ta yawan shan magunguna don cutar sankara ko kumburin da yake samar da insulin.
Abin da kuke buƙatar shirya don bincike
Don nazarin fitsari zaka buƙaci:
- Tsabtace, amma ba lallai ba ne tukunyar tarin fitsari mai tarin ruwa - gilashin gilashi ko gangar magani. Kada a lanƙwasa tsararren gwajin. Idan mara lafiyar na bushewa kuma babu isasshen fitsari, kuna buƙatar shirya babban daskararre.
- Adon robar da ba a shafa ba ko takarda bayan gida don yin jigilar gwajin gwajin.
- Sanyashi tare da kayan gwaji tare da sikelin da aka buga akan sa.
Ana siyar da gwajin a cikin filastik ko kuma ƙarfe na ƙarfe, yawanci 50 kowannensu, amma akwai wasu fakiti. The tube yawanci filastik, m sau da yawa - takarda. Kowannensu yana da sinadaran tsarin kulawar-kimiyyar sarrafawa. Lokacin da zafi yayi yawa, masu girki sun lalace, saboda haka ana bayar da kariya ta danshi a cikin bututu. Ana buƙatar silica gel desiccant akan murfi ko a cikin wata jaka dabam. Bayan kowane amfani, dole ne a rufe kwandon a rufe don hana iska shiga. Ba tare da ɗaukar masana'anta ba, ba za a iya ajiye abubuwan gwajin fiye da awa ɗaya ba.
Gwajin gwaji na iya samun na'urori masu auna sigari guda biyu: don tabbatar da jikin jikin ketone da glucose. Sugar yana fitowa a cikin fitsari idan aikin koda ya lalace ko kuma a cikin ciwon sukari lokacin da jininsa ya wuce 10-11 mmol / L. Akwai matakan gwaji na kasuwanci don hadaddun bincike na fitsari, waɗanda suke da abubuwan firikwensin har 13, gami da yunƙurin acetone.
Mahimmin yanayin yanki na abin mamaki shine sosai. Yana canza launi lokacin da ketones a cikin fitsari kawai 0.5 mmol / L ne. Matsakaicin matakin ganowa shine 10-15 mmol / l, wanda yayi dace da ƙari uku a cikin nazarin dakin fitsari.
Farashin gwajin acetone
Kudin kayan gwaje-gwajen da suka wajaba don nemo jikin ketone a cikin fitsari baya haɗa da farashin bayarwa idan kun siya su a cikin kantin magani ta kan layi. Kudin na iya bambanta sosai dangane da wurin da aka sayi alamu, lambobinsu a cikin kunshin ɗaya da ƙasar masana'anta.
Kimanin farashin kwatancen gwajin (manyan canje-canje na yiwuwa):
- a Rasha - daga 90 zuwa 1300 rubles a kowane kunshin,
- a cikin Ukraine - daga 30 zuwa 420 hryvnias,
- a Kazakhstan - daga 400 zuwa 6000 tenge,
- a cikin Belarus - daga 22,400 zuwa 329,000 na Belarusian rubles,
- a cikin Moldova - daga 25 zuwa 400 lei,
- in Kyrgyzstan - daga 100 zuwa 1400 som,
- a Uzbekistan - daga kasha 3,500 zuwa 49,000,
- a Azerbaijan - daga 2 zuwa 19 manat,
- a Armenia - daga 600 zuwa 8600 drams,
- a Jojiya - daga 3 zuwa 43 GEL,
- a Tajikistan - daga 9 zuwa 120 somoni,
- a cikin Turkmenistan - daga 4.2 zuwa 60.5 manat.
Umarnin don amfani a gida
Don yin amfani da tsaran gwajin don ƙaddarar acetone a cikin fitsari da kuma fassarar daidai sakamakon da aka samu, ba a buƙatar ilimin likita, cikakken isasshen bayani daga wannan labarin. Hakanan wajibi ne don nazarin umarnin takarda wanda aka haɗo shi a cikin kwali. Wasu masana'antun sun banbanta da lokacin bayyanar mai nuna alama a cikin fitsari da kuma lokacin da ake buƙata don canza launi na tsiri.
Likita na Kimiyyar Kimiyya, Shugaban Cibiyar Nazarin Diabetology - Tatyana Yakovleva
Na yi shekaru da yawa ina nazarin ciwon sukari. Yana da ban tsoro yayin da mutane da yawa suka mutu, har ma da yawa suna zama masu rauni saboda cutar sankara.
Na yi hanzarin ba da labari mai daɗi - Cibiyar Binciken Endocrinological na Kwalejin Kimiyya ta Rasha ta sami nasarar haɓaka maganin da ke warkar da ciwon sukari gaba ɗaya. A yanzu, ingancin wannan magani yana gab da kashi 98%.
Wani albishir: Ma'aikatar Lafiya ta tabbatar da ɗaukar wani shiri na musamman wanda zai biya diyyar magunguna mai yawa. A Rasha, masu ciwon sukari har sai 18 ga Mayu (m) samun shi - Don kawai 147 rubles!
Tsarin aiki
- A tattara fitsari a cikin akwati da aka shirya. Bai kamata ya zama yana da mayukan sukari, soda, sabulu ko kayan maye. Kafin bincike, yakamata a adana fitsari bai wuce awa 2 ba. Kuna iya ɗaukar kowane yanki na fitsari, amma mafi yawan nazarin da safe. Dangane da umarnin, ƙarancin adadin fitsari shine 5 ml. Idan bincike ba a yi shi nan da nan ba, kayan don an ajiye su a wuri mai duhu a ɗakin zazzabi. Fitsari ya cakuda shi kafin sanya tsirin gwajin a ciki.
- Cire tsiri gwajin, rufe bututu da ƙarfi.
- Rage tsagewar gwajin a cikin fitsari tsawon dakika 5, tabbata cewa dukkan manuniya sun dace da shi.
- Cire tsiri gwajin ka sanya gefen sa a kan adiko na goge baki don cire yawan fitsari.
- Don mintina 2, sanya tsirin gwajin a kan busasshiyar ƙasa tare da masu firikwensin sama. A wannan lokacin, halayen sunadarai da yawa zasu gudana a ciki. Idan akwai acetone a cikin fitsari, firikwensin don tabbatar da dalilinsa zai canza launi.
- Kwatanta launi na firikwensin tare da sikelin wanda ke kan bututun kuma ƙaddara matakin ƙaddara na jikin ketone. Strongerarfin ƙarfi da launi, mafi girman taro na acetone.
Don samun sakamako na abin dogara, ana aiwatar da binciken ne a zazzabi na 15-30 ° C. Binciken zai zama ba daidai ba idan an adana fitsari tsawon lokaci ko a fenti a wani launi mai haske. Dalilin wannan toshewar na iya zama wasu magunguna da abinci, kamar beets.
Fassara sakamakon:
Jikin Keto, mmol / l | Yarda da urinalysis | Bayanin |
0,5-1,5 | + | Might acetonuria, ana iya warke kanta. |
4-10 | ++ | Matsakaicin digiri. Tare da shan yau da kullun, fitar da fitsari na yau da kullun da kuma rashin matsanancin ƙwayar ciki, zaku iya shawo kan sa a gida.Yara ƙanana da marasa lafiya da ke ɗauke da sukari mai yawa na iya buƙatar taimakon likita. |
> 10 | +++ | Mai tsananin mataki. Ana buƙatar asibiti mai gaggawa. Idan kuma an gano cutar glucose mai yawa a cikin fitsari, kuma yanayin mai haƙuri ya kara yin muni, tofinan mahaifa zai yiwu. |
Inda zaka siya da farashin
Kuna iya siyan tsaran gwajin don kasancewar acetone a cikin kowane kantin magani, takardar sayen magani ba dole bane. Lokacin sayen, kula da ranar ƙarewa, kafin ƙarshensa ya zama fiye da watanni shida. Wannan shine yawan alamu riƙe aikin su bayan buɗe kunshin.
Rarraban hanyoyin gwaji a cikin magunguna a Rasha:
Manuniya | Alamar kasuwanci | Mai masana'anta | Farashin kowace fakiti, rub. | Adadin kowace fakiti | Farashin 1 tsiri, rub. |
Jikin Ketone kawai | Ketofan | Lahema, Czech Republic | 200 | 50 | 4 |
Uriket-1 | Biosensor, Rasha | 150 | 50 | 3 | |
Ketones na bioscan | Bioscan, Russia | 115 | 50 | 2,3 | |
Jikin Ketone da glucose | Ketogluk-1 | Biosensor, Rasha | 240 | 50 | 4,8 |
Bioscan glucose da ketones | Bioscan, Russia | 155 | 50 | 3,1 | |
Diaphane | Lahema, Czech Republic | 400 | 50 | 8 | |
5 sigogi, gami da ketones | Penta na Bioscan | Bioscan, Russia | 310 | 50 | 6,2 |
10 suturar fitsari | UrineRS A10 | Babban Fasaha, Amurka | 670 | 100 | 6,7 |
Alamar Tausasawa 10EA | Arkrey, Japan | 1900 | 100 | 19 | |
Manuniya 12 na fitsari ban da acetone | Dirui h13-cr | Dirui, China | 950 | 100 | 9,5 |
Bugu da ƙari, zaku iya karanta:
Tabbatar koya! Shin kuna tsammanin kulawa da kwayoyin hana daukar ciki da insulin shine hanya daya tilo da za'a kula da sukari? Ba gaskiya bane! Kuna iya tabbatar da wannan da kanku ta hanyar fara amfani da shi. kara karantawa >>
Menene gwajin acetone?
Don sauri gano ketones a cikin fitsarin mutum, ana amfani da alamun gwaji wanda kowa zai iya siyan kansa da kansa a kusan kowane kantin magani. Don yin wannan, ba kwa buƙatar tuntuɓar ƙwararru tare da ƙari, alal misali, don takardar sayen magani.
Takaddun gwajin don ƙaddarar acetone a cikin fitsari ana samun su a cikin kwantena waɗanda aka yi da filastik da ƙarfe, ko a cikin ƙananan gilashin gilashi. A cikin kunshin ɗaya na iya zama daga guda biyar zuwa 200 na tube. Kowane mai nunawa an yi shi ne da lilin kuma aka sanya shi cikin wani abu na musamman wanda ke taimakawa wajen tantance acetone a cikin fitsari.
Wace hanya aka bayyana don gano ketonuria?
Bayyan acetone a cikin fitsari alama ce ta firgita, wanda da farko tana buƙatar shawara ta gaggawa na ƙwararrun masaniyar endocrinologist. Abu ne mai sauki a tantance wannan yanayin ta hanyar warin da ke tattare da numfashi da fitsari da shi. Ana gudanar da cikakken binciken kwalliya da kuma matakan da suka dace na magani a cikin ma'aikatar lafiya.
An tsara matakan gwaji don auna matakin kwayoyin mahadi a jikin mutum - samfuran matsakaici na mai, carbohydrate da metabolism metabolism. An dauki su a matsayin kayan aiki mafi inganci don tantance matsayin acetonuria. Abubuwan gwaji alamun nunawa ne na yawan ketones a cikin fitsari.
An adana su a gilashin, karfe ko filastik kuma ana samun su don siyarwa kyauta a cikin sarkar kantin magani - ana siyar dasu ba tare da takardar sayan magani ba. Packageaya daga cikin kunshin zai iya ƙunsar daga gwaji 50 zuwa 500. Don bincika abubuwan da ke tattare da jikin acetone a cikin fitsari, ana bada shawarar siyan kunshin tare da mafi yawan adadin gwaji.
Kafin amfani, suna da fari, gefen su yana cike da kayan reagent na musamman (nitroprusside sodium). Bayan an tuntuɓar da ƙwayar ƙwayar cuta, wannan abu yana canza launi; don karanta bayanan gwaji na ƙarshe, umarnin tsarin bayyanar ya ƙunshi sikelin launi da tebur don warware sakamakon.
Shahararrun hanyoyin binciken cutar sauri sune:
Shiri da dokoki na binciken
Umarnin don yin amfani da alamun gwajin nuna alama na iya bambanta dangane da masana'antun su, amma bukatun na yau daya suke. Ana gudanar da binciken a zazzabi + + zuwa + 28 ° C. Guji taɓa hannayenku tare da sassan abubuwan gwaninta na kayan gwaji.
Yi amfani da sandunan da aka cire daga cikin akwati na minti 60. Ya kamata a tattara samfurin fitsari a cikin ganga mai ruwa. Don gwajin, ana amfani da ruwan ɗimbin da aka tattara. Don sanin ƙimar ketonuria, dole ne a aiwatar da waɗannan matakai:
- saka safofin hannu na likita
- ɗauki gwajin a fili daga kunshin kuma rufe murfin sake akai.
- na dan lokaci kaɗan, ka rage gefen manuniya a cikin fitsarin da aka tattara (kusan 10 ml ya isa),
- a hankali cire karin ruwan jiki tare da bushe bushe,
- Sanya sandar gwajin a kan tsaftataccen fili tare da abun taɓawa sama,
- bayan minti 2-3, gwada sakamakon gwajin tare da sikelin akan kunshin.
Ka'idar nazarin fitsari ta amfani da tsinkewar gwaji ya dogara ne da halayen launi na Shari'a, wanda ɓangaren ɓangaren nuna alama ke haɗuwa da fitsari yana ɗaukar launin shuɗi.
Fassara Sakamako
Abubuwan da aka dogara dasu sune bayanan karshe na saurin gano cutar sikelin ketonuria wanda aka yi a cikin nazarin sashen fitsari safe. Don kimanta sakamakon gwajin, kuna buƙatar kwatanta launi na gefen tsiri tare da sikelin da aka gani akan kunshin.
Sanadiyyar shigowar inuwa daga cikin kayan nuna alama ana bada shawarar yin nazari a cikin haske mai haske. Mafi ƙarancin ketones a cikin fitsari shine 0.5 mmol / l, mafi girma shine 15.0. Gwajin saurin ya ba da damar gano gawar ketone kawai, har ma don sanin matsayin karuwar su.
Sakamakon binciken ya kasu kashi biyu:
- Babu bincika gefen alamar tsiri - sakamako mara kyau, wanda ke nuna rashin acetone a cikin fitsari.
- Haske mai ruwan hoda mai haske yana nuna matsayin mai sauƙi na ketonuria. Wannan yanayin ba shi da haɗari ga rayuwar ɗan adam, amma yana buƙatar cikakken bayyanin ganewar asali.
- Cikakken ruwan hoda da launin rasberi yana bayyana sakamakon adadi da yawa na jikin ketone - yana nuna matsakaicin darajar acetonuria, yana buƙatar magani nan da nan.
- Launin violet na gwajin gwaji ya samu tare da keto-acidosis - babban matakin ketone a cikin fitsari. Halin yana haifar da barazana ga rayuwar mai haƙuri kuma yana buƙatar asibiti a asibiti.
Idan kun sami sakamakon m na bayyanar cututtuka (canje-canje inuwa ba daidaituwa ba ne ko kuma faruwa bayan mintina 5), dole ne ku sake maimaita gwajin. Zai dace a bincika gaskiyar cewa wasu kwayoyi na iya shafar sakamakon binciken. Abin da ya sa, bayan gudanar da shi kan kanku, ya kamata ku tuntuɓi ƙwararrun ƙwararrun masani don cikakken bincike.
Muhimmancin Kula da Kai
Tsawon lokaci acetonuria yana ba da gudummawa ga abin da ya faru na cutar kansa, cututtukan da ke cikin jijiya da kwakwalwa. Yana da matukar muhimmanci ga yara, uwaye masu fata da kuma masu fama da cutar siga don sarrafa adadin ketones a cikin fitsari. Dole ne a bayar da gwaji don gano karuwar su lokacin da:
- tsananin ciwon kai, tashin zuciya, da amai
- zazzabi
- general malaise
- rashin ci.
Alamomin da aka jera na iya zama alamun asibiti na lalacewar aiki na tsarin juyayi ko yawan zazzabi a cikin tattarawar glucose a cikin jini. Binciken fitsari wanda ba a sani ba na iya juyawa zuwa cikin haɓakar haɓakar cuta kuma zai haifar da rikitarwa mai rikitarwa, rikicewar tsarin jijiyoyi, raguwa mai kaifi a matakan sukari da cutar ciwan-ciki.
Koyaya, ya kamata a tuna cewa ba shi yiwuwa a yi gwaji kuma a gwada maganin! Don hana faruwar abin da ke faruwa na cututtukan cututtukan cuta, kuna buƙatar cin abinci daidai, lura da tsarin shan giya, kar ku ci barasa kuma ku rarraba ayyukan jiki.
Semi-adadi ƙaddara na gwaji gwaji
Ana gudanar da binciken ƙimin-ƙima yayin aiwatar da sakamakon kuma ya ƙunshi ƙirƙirar wasu ƙarar jikin ketone a cikin fitsari ta hanyar amfani da hanyar karin launi na alamomin gwaji, kuma tare da sikelin launi na musamman, wanda, a matsayinka na doka, ana iya samo shi a kan kunshin tare da kwalliyar gwaji.
Gwajin gwajin kwayoyin cutar mahaifa
Determinationudurin jikin ketone a cikin fitsari ta hanyar amfani da gwajin acetone a cikin fitsari ya dogara ne akan gwajin doka. Yayin aiwatarwa, ana aiwatar da sakamako tsakanin sodium nitroferricyanide da lu'u-lu'u (sune ɓangarori ne na alamar tsiri gwajin).
Sakamakon haka, motsin alamar mai gwajin ya sami launi mai launin shuɗi a cikin wata ko wata inuwa, wanda yake daidai da adadin ketone jikin da ke cikin fitsari. Yankin azanci na yawancin gwaje-gwajen acetone ana kiyaye shi daga ascorbic acid.
Magunguna, har da magunguna da aka yi amfani da shi don gano asali, na iya haifar da sakamako na rashin gaskiya ko na karya. Sakamakon bincike, wanda ba shi da cikakken ko gaba ɗaya bai dace da hoton da ke gudana ba, dole ne a bincika ta amfani da sauran hanyoyin bincike.
Ya kamata a maimaita gwajin acetone a cikin fitsari bayan an gama maganin.
- Carriedarfafa yawan haɗarin ketone a cikin fitsari ana yin shi ne tsakanin kewayon daga 0.0 zuwa 16 mmol / L, ƙaramin abun ciki na jikin ketone shine kusan 5 mmol / L.
- Girman launi (yana iya kasancewa a cikin tsarin tabular), ana samarwa a kan kunshin tare da tsararran gwaji, ya haɗa da sassan launi guda shida waɗanda ke dacewa da takamaiman ketone.
Gwajin gwaji
An tsara gwajin mai nuna alama ne don binciken kai-tsaye game da fitsari, don amfani da shi, ba kwa buƙatar samun ilimin likita na musamman ko gogewa.
Takaddun gwaji don ƙaddarar acetone a cikin fitsari an ƙirƙira su ne a cikin 1941 ta Dr Miles. Wannan manuniya shine gyara na Benedict reagent, wanda aka fara keɓance shi musamman da nau'in ruwa, sannan kuma a cikin allunan.
A zahiri, allunan sune nau'in bushewar bushewar farko da ake buƙata don ƙayyade adadin glucose a cikin fitsari na marasa lafiya da masu ciwon sukari mellitus. Aikace-aikacen da allunan da aka yi a lokaci guda sun ci gaba har zuwa ƙarshen ƙarshen ayyukan.
Sakamakon bincike da aka yi ta amfani da gwajin acetone na iya dogara da waɗannan abubuwan:
- concentarin maida hankali ne ascorbic acid,
- acid, wanda samfuri ne na hadawan abu da iskar shaka,
- magunguna
- ragowar na maye gurbi da masu tsabtace waɗanda aka yi amfani da su don tsaftace ɗakunan tattara fitsari.
Umarnin don amfani da gwajin acetone a cikin fitsari
Yin nazarin umarnin don amfani da gwaje-gwajen acetone a cikin fitsari a nan ba ya rage maka karanta umarnin a cikin kunshin tare da tsaran gwajin da zaku saya.
Umurnai don amfani da waɗannan alamun suna iya bambanta cikin abun ciki da shawarwari dangane da mai ƙirar gwajin:
- Ya kamata a aiwatar da ma'auni a zazzabi na daga goma sha biyar zuwa talatin.
- Babu buƙatar taɓa ɓangaren firikwensin, ya kamata ku tuna game da ƙa'idodin ƙa'idodin tsabta.
- Bayan an cire tsiri na gaba daga kunshin, dole ne a rufe shi nan da nan tare da murfi.
- Don nazarin, ana amfani da fitsari sabo (wanda aka samo ba sa wuce sa'o'i biyu da suka gabata), gauraye, ba tare da adana magunguna kuma a cikin ganga mai bakararre. Wannan kwandon bai kamata a fallasa shi da hasken rana kai tsaye ba.
- Ana iya samun sakamako mafi daidaitaccen bincike da safe.
- Capacityarfin da za'a tattara fitsari kada ya ƙunshi abubuwan tsabtatawa da masu sha.
- Idan samfurin fitsari ya yi duhu sosai kuma yana cike da ƙazanta, zai yi wahala sosai a fassara sakamakon binciken daidai.
- Mafi ƙarancin abin da zai yiwu a aiwatar da bincike shine milili biyar na fitsari.
Don haka, ya kamata a nutsar da tsirin gwajin a cikin isasshen fitsari, ko kuma a yi amfani da abin sha don gwajin.
Bayan shirya, zaku iya ci gaba kai tsaye zuwa binciken:
- Bude kunshin kuma cire tsiri,
- Rufe murfin nan da nan,
- Nutsar da mai nuna fitsari a cikin dakika biyu,
- A fitar da gwajin
- Cire yawan fitsari tare da adiko na goge baki ba tare da an nuna mai da kanta ba,
- Sanya tsiri a ɗakin kwana, busasshiyar ƙasa tare da mai nuna sama,
- Bayar da sakamakon da aka gama ba a farkon minti biyu bayan fara binciken ba, kwatanta launi na mai nuna alama tare da ma'aunin launi akan kunshin.
Sakamakon binciken:
- 0.5 mmol / l zuwa 1.5 mmol / l- tsananin rauni. Ana iya kula da ku da kanku a gida,
- 4 mmol / L - matsakaici mai ƙarfi. Idan aka lura da wannan yanayin a karon farko, to babu damar a shayar da mara lafiya, kuma yanayin lafiyarsa yana ta yin muni kuma yai muni, kuna buƙatar ganin likita,
- Mataki 10 mmol / L - Mai tsananin. Ana buƙatar asibiti cikin gaggawa.
Siffar launi
Kowane masana'anta na kayan gwaji, ma'aunin launi da ke kan kunshin, ya bambanta da adadin filayen da kuma ƙarfin inuwa. A kan hanyar sadarwa za ku iya samun jerin duk hanyoyin gwajin da aka rarraba.
- Arina Na sayi bututun gwaji na Bayer, farashin yana da sauƙin araha a gare ni, ya dace kuma mai sauƙin amfani, sakamakon yana farantawa daidai. Ina bayar da shawarar shi!
- Sergey Na sayi kayan Uriket, komai dacewa, banda guda ɗaya - wani lokacin abu ne mai wuya kawai ku same su a cikin kantin magunguna a garin na! Wannan a sarari yake mummunan fahimta a gare ni.
Acetone a jiki
Aketone mai yawa a cikin jini yana bayyana lokacin da tsarin ƙwayoyin cuta ya daina jurewa da kawar da samfuran rabin abubuwa na mai, furotin da abubuwan gina jiki. Saurin acetone yana haɗuwa cikin jiki, da sauri dukkanin ƙwayoyin sun lalace, kuma a farkon, ƙwayoyin kwakwalwa.
Jiki yana rasa ruwa, tafiyar matakai na rayuwa sun lalace. A wannan yanayin, yakamata a gudanar da gwajin acetone da wuri-wuri, saboda saurin haɓakar cutar na iya haifar da rashin lafiya.
Aboutari game da hanyar bayyana
A cikin ɓangaren na'urar na'urar lafiya, sandunan gwaje-gwaje don bincika acetone a cikin fitsari ana kiransu "hadaddun ƙwayoyin bincike." A cikin yanayin da ba a tsaye ba, ana amfani da saiti na yau da kullun wanda ya ƙunshi daga takarda 5 zuwa 100 ko kuma sandun filastik mafi yawan lokuta tare da nuna alama. An tattara su a cikin akwati na takarda na musamman kuma ana sayar dasu a cikin kantin magani ba tare da takardar sayan magani ba. An haɗa da dehumidifier na roba a cikin akwatin alamar don hana danshi daga kafawa.
Ana amfani da tsaran gwaje-gwaje don ƙaddarar acetone a cikin fitsari don ingancin da kuma ƙididdigar ƙididdiga. Dogaro da gyararraki da mai ƙera kaya, yana yiwuwa a bincika tare da taimakon jikinsu don abubuwan da ke tattare da jerin abubuwa. Binciken ƙididdiga ya nuna gaskiyar kasancewar ɓangaren, yayin ƙididdigar ƙididdiga ta ƙunshi bayanai akan matakin sa.
Ana amfani da reagent (sodium nitroprusside) a kowane tsiri, wanda, ya danganta da ketone a cikin fitsari, ana canza launin launuka daban-daban. Don karanta sakamakon gwajin, umarnin sun ƙunshi teburin rubutu da rubutu. Ana nuna matakin acetone ta hanyar giciye ko ƙari.
Indexarfin ma'aunin haske yana ƙaruwa a cikin daidaito kai tsaye zuwa adadin abubuwan ketone.
Mahimmanci! A gaban manyan cututtuka, bayyanar cututtuka tare da raunin gwaji ba ya maye gurbin bayar da gwaje-gwaje na dakin gwaje-gwaje na fitsari na yau da kullun, amma yana aiki ne kawai a matsayin hanyar bayyana kimanta yanayin.
Dokoki don amfani da tube
A daidai da umarnin, ana buƙatar a kalla 5 ml na fitsari don gwajin. Wani fifiko shine tsabtar ruwan halittar jiki, daga lokacin tattarawa bai wuce mintuna 120 ba su wuce. Adana lokaci mai tsawo yana ƙara yawan acidity kuma yana haifar da gurbata sakamako.
Don gano daidai ketone jikin, ba za a yarda da abubuwa na ƙasa da ruwa cikin fitsari ba. Yakamata a tattara allurar a bakararre kuma ta girgiza ko gauraya kafin gwaji.Ya kamata a kiyaye ikon daga hasken rana da matsanancin zafi ko zafi. Bugu da kari, don samo bayanan abin dogara, dole ne a kiyaye wadannan bukatu:
- Ana yin gwajin fitsari cikin sauri a cikin ɗaki tare da yawan zafin jiki na ƙasa ba ƙasa da +15 ba kuma ya fi +30,
- haramun ne a taba wurin aikace-aikace a kan tsiri na reagent
- An bada shawara don bincika sashen fitsari na safe,
- Idan ana tattara mata, wajibine a hana shigowa da jinin haila da zubar farjin,
- Kafin yin kumburi, kada kuyi amfani da samfuran tsabta don wanka (ruwa mai tsabta).
Ya kamata a cire matakan acetone a cikin fitsari a cikin shari'ar fensir nan da nan kafin aikin. Rufe akwatin nan da nan don hana danshi daga ciki.
Manunin dole ne a tsoma shi cikin ruwan halittun har sai an rufe shi gaba daya. Riƙe kamar secondsan seconds. Yi amfani da bushe bushe don cire ɗimbin saukad daga kullu, ta hanyar jika a hankali, ba tare da taɓa yankin tare da reagent ba. Na tsawon dakika 120, an sanya tsiri akan tebur bushe ko kanti tare da alamar nuna sama. Bayan lokacin amsawa, yanke shawarar matakin acetone ta hanyar amfani da sanda a cikin tsarin launi. Zai fi kyau a yi wannan da rana.
Bayyana sakamakon
Ana nuna alamun karatu daidai da alamar akasin inuwa da ake so.
Daraja | Matakan jikin ketone da 100 ml |
---|---|
Debe (-) | 0 (babu acetone). |
Debe kuma da (- +) | Har zuwa 5 MG (al'ada). |
Karin (+) | Babu fiye da 10 MG (m digiri na acetonuria) wanda zai iya yin jiyya a cikin yanayin da ba shi da lafiya. |
Plusari biyu (++) | Har zuwa 40 MG (yanayin da ke kusa da matsakaici) yana buƙatar mai aikin outpatient ko inpatient treatment. |
Plusari uku (+++) | 100 da mafi girma MG (acetonuria mai tsanani), barazanar lalacewar kwakwalwa da haɓaka ƙwayar cuta. Jiyya kawai a asibiti, wani lokacin a cikin sashin kulawa mai zurfi. |
Ya danganta da kamfanin da ke kera kaya, ragi don tantance ketones a cikin fitsari na iya wasu lokuta suna da sikeli daban-daban don kimanta sakamako da adadi mara kyau na alamomin launi. Lokacin gudanar da gwaji don acetonuria, ana aiwatar da karatun bayanan bincike ne bisa ga umarnin "'yan ƙasa" da aka haɗa da akwatin.
Hankali! Harkokin magani na roba na iya shafar ƙaddarar acetone a cikin fitsari, yana haifar da matsanancin ƙarar mai nuna alama kuma, a sakamakon haka, sakamakon ƙarya. Sabili da haka, dole ne a yi gwajin tsakanin darussan magani.
Yanayin ajiya
Dole ne a kiyaye samfuran a cikin sandar bushewa ko majalisa a zazzabi na +2 zuwa +30. Kada kabar abubuwan danshi ko abubuwan sinadarai su shiga kunshin. Kiyaye tsummoki a firiji, an hana su, kuma kada su isa ga yara.
Rayuwar shiryayyen akwati wanda ba a buɗe ba ya kai har zuwa shekaru 2, ya danganci mai sana'anta. Za'a iya amfani da buɗaɗɗiyar buɗewa tare da kullu bai wuce wata shida ba. Abubuwan da aka yi amfani da alamun nuna alama basu dace da sake yin gwaji ba. A cikin asibiti, ana karɓar su kamar ƙarancin kamuwa da cuta na aji "B" kuma zubar da su.
Matsalar gwaji a cikin launi wanda ba a nuna kan sikelin na iya zama alamar nuna gazawar saboda ƙarewar lokacin karewa ko ajiyar da ba ta dace ba.
Iri daban-daban na ratsi da farashi
Gwajin kai tsaye don auna acetone a cikin ruwan jiki ya sha bamban. Zasu iya samun kwanakin karewa daban-daban, ka'idodi na gudanar da binciken, kuma sun banbanta cikin yanayin karanta sakamakon. Akwai gwaje-gwaje da aka ƙera don auna gwargwadon ƙwayoyin ketones kawai, kuma akwai wasu hanyoyin don tantance abubuwa da yawa a cikin fitsari.
Adadin alamu da nau'in abun da ake ƙaddara | Sunan, mai kullu da farashi guda 50 |
---|---|
1 - acetone. | Ketofan (Lachema, Czech Republic) 202 rubles, |
Uriket (Biosensor, Russia) 164 rubles,
Ketones Bioscan (Bioscan, Russia) 130 rubles.
Kwayar halittar “Glucose Ketones” (Bioscan Russia) 170 rubles.
Penta na bioscan (Russia, Bioscan) 310 rubles,
Uripolian -11 (Biosensor, Russia) 780 rubles.
Kudin sanannun tsarukan gwaje-gwaje na acetone a cikin fitsari kai tsaye ya dogara da tsarin masu nuna alama. Kuna iya siyan samfuran a kowane kantin magani na kan layi ko kan layi.
Hankali! Lokacin sayen alamu, kuna buƙatar bincika marufi a hankali don mutuntawa kuma ku kula da ranar karewa. Ya kamata a kirkiri adadin hanyoyin da ake buƙata a gaba don kar su watsar da waɗanda ba a amfani da su ba saboda jinkiri.
Gwajin gida bai maye gurbin cikakken nazarin dakin gwaje-gwaje na fitsari ba kuma yana iya samun ƙarancin ƙididdigewa, amma yana da matukar mahimmanci idan saka idanu akan abubuwan jikin ketone a cikin jiki ya zama dole. Binciken yana taimaka wajan tantance yanayin abubuwan da ake cin abinci na dogon lokaci da cututtuka na rayuwa. Ikon auna acetone a cikin fitsari tare da tsinkewar gwaji ba tare da barin gida ya ba da damar masu cutar sukari su guji cutar sikari ba, kuma ga mata masu juna biyu masu rikitarwa. Babban fa'idar hanyar ita ce sauki, saurin magana da ƙwarewar cutar kansa ba tare da kasancewar ƙwarewa na musamman ba.
Menene acetone kuma a ina yake cikin fitsari
Hankalin mutum yana yin ɗumbin yawaitar glucose kowace rana. Wannan tsari yana haɗuwa tare da samuwar a cikin jikin ketone, wanda ya haɗa da acetone da nau'ikan acid biyu. A al'ada, suna kasancewa a cikin fitsari a cikin ƙaramin abu, har zuwa 2 ko 5 a kowace mil 100 kuma kusan ba a nuna su a cikin sakamakon bincike mai sauri.
Tare da rikicewar metabolism, wanda akwai ƙarancin aiwatar da rushewar sukari tare da haɓaka haɓakar mai da furotin, matakin acetone a cikin ruwa mai narkewa yana ƙaruwa. Zai fara zama a cikin fitsari, kuma yanayin cutar na faruwa - ketonuria.
Lura! Hadarin acetone ga mutane ba shine ainihin alamar kasancewar sa cikin fitsari ba, amma a cikin haɓakar ƙwayoyin cuta a matakin halatta. Yawanta mai yawa a cikin jiki ya cutar da duk mahimman tsarin, musamman ƙwayoyin kwakwalwa.
Sanadin da bayyanar cututtuka na acetonuria
An kirkiro sinadarin ketones a cikin fitsari yayin da tsarin urinary bazai iya magance fitowar kayayyakin lalatattun abubuwa na glucose, furotin da mai ba. An sauƙaƙe wannan ta hanyar cututtukan hormonal da na rayuwa, gazawar Cardinal a cikin aikin gabobin ciki da tsarin endocrine.
Acetonuria yawanci alama ce ta tsarin tumo, acromegaly, ciwon sukari mellitus, cututtukan cututtukan cuta da kuma cututtukan hoto. Har ila yau yanayin yana tasowa daga yanayin rashin cin abinci mai wahala, yawan aiki, abinci mai gina jiki da yawan wuce kima ko sarrafa insulin.
Kasancewar cututtukan acetone a cikin jiki yana cutar da mucous membranes na ciki da hanji, yana shafar jijiya da ƙwaƙwalwar kwakwalwa, a cikin manyan lokuta yana iya tayar da komputa, gazawar zuciya da guba da kansu. Yin gwajin ketone ya zama dole yayin da alamun cututtukan da ke gaba suka haɓaka, musamman idan yana tare da iskar acetone:
- amai
- zafi a ciki da kewayen cibiya,
- tashin zuciya
- rage cin abinci
- migraine ko ciwon kai
- apathy da lethargy,
- tsananin farin ciki.
Yara na iya bugu da haveari suna da zazzabi. Halin yakan haifar da rashin ruwa, yawan maye kuma yana fuskantar barazanar rayuwa. A wannan yanayin, rikicewar metabolism yana ci gaba da sauri. Gano matakan tsayi na ketones a cikin fitsari na mata masu juna biyu yana nuna yiwuwar take hakki a cikin aikin gabobin endocrine. Mafi yawan lokuta suna tsokani da ci gaban tayin da kuma kara damuwa akan jikin mace.
A cikin mawuyacin yanayi, tare da babban nauyin maye da barazanar lalacewar ƙwayoyin kwakwalwa, an dakatar da daukar ciki na wani ɗan gajeren lokaci, kuma a ƙarshen lokacin, ana haifar farkon haihuwa.
Ketones a cikin fitsari
Ketones a cikin fitsari na mutane da ciwon sukari suna nuna ƙarancin aiki a cikin aikin mutum. Acetone a cikin fitsari a gaban ciwon sukari yana da nisa daga bayyanuwar wannan cutar, saboda koyaushe yana tare da haɓaka yawan haɗuwa da glucose a cikin jini.
A cikin cututtukan cututtukan fata, fitsari alama ce ta kusan dukkanin cututtukan da ke cikin jikin mutum. Kasancewar kasancewar ketones a cikin fitsari shine shaidar farko ta cutar da ba'a sani ba.
Gwajin Ketone
Sai kawai tare da alamar acetone:
- Uriket (masana'anta - Russia),
- Cytolab (masana'anta - Ukraine),
- Ketostix (masana'anta - Jamus),
- Ketofan (masana'anta - Czech Republic),
- DAC (masana'anta - Moldova).
Manuniya guda biyu (sukari da ketones):
- Ketogluk (masana'anta - Russia),
- Diafan (mai samarwa - Czech Republic).
Manuniya uku ko sama (sukari, ketones, jini da yake ɓoye, cikakken furotin, da sauransu):
- URS (masana'anta - Jamus),
- Dekafan (masana'anta - Czech Republic),
- Pentafan (masana'anta - Czech Republic).